454 GS FLX Titanium sequencing - SRA

ERX1267224: 454 GS FLX Titanium sequencing
1 LS454 (454 GS FLX Titanium) run: 111,604 spots, 45.8M bases, 111.9Mb downloads

Submitted by: 454 LIFE SCIENCES/RO (454 Life Sciences/Ro)

Study: Multicenter study to validate an updated assay design for next-gen sequencing to identify HIV-1 drug-resistance mutations and determine co-receptor use with high sensitivity

PRJEB12165 • ERP013607 • All experiments • All runs

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Ultra deep sequencing is of increasing use not only in research but also in diagnostics. For implementation of ultra deep sequencing assays in clinical laboratories for routine diagnostics, intra- and inter-laboratory testing are of the utmost importance. A multicenter study was conducted to validate an updated assay design for 454 Life Sciences’ GS FLX Titanium system targeting protease/reverse transcriptase (RTP) and env (V3) regions to identify HIV-1 drug-resistance mutations and determine co-receptor use with high sensitivity

Sample: 2nd HIV UDS aphastudy

SAMEA3715154 • ERS1022303 • All experiments • All runs

Organism: HIV-1 M:B

Library:

Name: unspecified

Instrument: 454 GS FLX Titanium

Strategy: AMPLICON

Source: VIRAL RNA

Selection: RT-PCR

Layout: SINGLE

Runs: 1 run, 111,604 spots, 45.8M bases, 111.9Mb

Run	# of Spots	# of Bases	Size	Published
ERR1193911	111,604	45.8M	111.9Mb	2015-12-19

ID:: 2114316

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